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Normally enzymes are present in cells in rather low concentrations.
4. Substrate concentration
The rate of an enzyme-catalysed reaction shows a curved dependence on substrate concentration. As the substrate concentration increases, the rate increases because more substrate molecules can collide with enzyme molecules, so more reactions will take place...
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At higher concentrations the enzyme molecules become saturated with substrate, so there are few free enzyme molecules, so adding more substrate doesn't make much difference (though it will increase the rate of E-S collisions).
The maximum rate at infinite substrate concentration is called vmax, and the substrate concentration that give a rate of half vmax is called KM...
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