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Topic: Science
Number of pages / Number of words: 4 / 1071
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Mammalian cell lines expressing the BCRP-G exhibit an enhanced efflux of cationic anthracyclines and rhodamine 123, but a decreased efflux of anionic methotrexate when compared with cell lines expressing BCRP-R. Experiments were performed to test whether these differences in drug selectivity of BCRP-G and -R were retained in L...


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lactis. The experiments showed that BCRP-G mediated the transport of rhodamine 123 and the rhodamine analogue TMR, but BCRP-R was unable to handle these substrates. The interaction of BCRP-G/R with methotrexate was studied in competition assays on the basis of the BCRP-mediated transport of the fluorescent substrate Hoechst 33342...


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