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Science
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| Number of pages / Number of words: |
8 / 2109 |
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Electronic energy levels exist because electrons in molecules can be excited to discrete, higher energy orbitals. The energy (E) of light depends on its wavelength. Longer wavelengths (infrared) have less energy than shorter wavelengths (ultraviolet). A molecule will absorb energy (light) when the energy (or wavelength) exactly matches the energy difference between the two energy states of the molecule...
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The light we see coming from that object is the light which was not absorbed by the object. We see the "not-absorbed" light as the color of the object. If no light is absorbed, the object appears to be colorless.
A spectrophotometer is employed to measure the amount of light that a sample absorbs. The instrument operates by passing a beam of light through a sample and measuring the intensity of light reaching a detector...
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quantitative analysis by spectrophotometric methods
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