|Number of pages / Number of words:||8 / 1981|
Results are expected to vary depending on the number and type of miRNA inserted. Additive effects, multiplicative effects and possible interference of the addition of one or more miRNAs will be looked for.
Materials and Methods
Cell culture and transfection
HeLa cells and HEK 293T cells were cultured in DMEM supplemented with 10% FBS...
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Transfections of various amounts of reporter plasmid coupled with the miR expression plasmid were carried out 24 hours after seeding 24/96 well plates using Lipofectamine 2000 (Invitrogen). Luciferase assays were carried out 24-48 hours after transfection using the Dual- luciferase assay system (Promega)...
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