Buy custom gene cloning
Essay's paper info
| Topic: |
Science
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| Number of pages / Number of words: |
2 / 448 |
Essay's paper body
Clone: A group of identical cells or organisms
To clone a gene:
1) Insert the gene into a vector that can carry the into a host cell
2) Ensure that it will replicate there
3) Insertion is carried out by cutting the vector and the DNA to be inserted with the same restriction endonuclease to ensure that both have the same "sticky ends"
Vector for cloning the bacteria come from two major types:
(1) Plasmids, and
(2) Phages
Recombinant DNA Technology
A) It was developed in 1970
B) Procedure:
(1) Obtain DNA from an organism
(2)ADD Restriction enzymes
(3) A reproducible set of fragments
(4)Inset into replicating DNA molecules
(5) This gives Recombinant DNA
(6) Introduce this DNA in appropriate cells
Why clone a gene?
Let's say you have a Genome with GeneA, GeneB & GeneC
Your gene of interest is GeneA, You would
(1) Select the gene of interest
(2) Clone the gene, which could be used for
a) DNA sequence, Protein product expression
b) Gene Therapy
c) Prepare the gene product in large scale
Restriction digestion generates a reproducible set of DNA fragments(?)
Restriction Enzymes:
1) Bacterial Enzymes that recognize a specific 4-8 base pair sequences in double stranded DNA
(2) Cleave both DNA strands at the restriction sites...
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Clone: A group of identical cells or organisms
To clone a gene:
1) Insert the gene into a vector that can carry the into a host cell
2) Ensure that it will replicate there
3) Insertion is carried out by cutting the vector and the DNA to be inserted with the same restriction endonuclease to ensure that both have the same "sticky ends"
Vector for cloning the bacteria come from two major types:
(1) Plasmids, and
(2) Phages
Recombinant DNA Technology
A) It was developed in 1970
B) Procedure:
(1) Obtain DNA from an organism
(2)ADD Restriction enzymes
(3) A reproducible set of fragments
(4)Inset into replicating DNA molecules
(5) This gives Recombinant DNA
(6) Introduce this DNA in appropriate cells
Why clone a gene?
Let's say you have a Genome with GeneA, GeneB & GeneC
Your gene of interest is GeneA, You would
(1) Select the gene of interest
(2) Clone the gene, which could be used for
a) DNA sequence, Protein product expression
b) Gene Therapy
c) Prepare the gene product in large scale
Restriction digestion generates a reproducible set of DNA fragments(?)
Restriction Enzymes:
1) Bacterial Enzymes that recognize a specific 4-8 base pair sequences in double stranded DNA
(2) Cleave both DNA strands at the restriction sites...
Essay fragment
General points of the essay
GENE ONE INC
Gene One
The Gene
Gene Therapy 3
Gay Gene
Gene Therapy Analysis
Gene and Genome
Gene-therapy: How will it Change the Future of Genetic Disorders
gene cloning
human gene therapy
Restriction Enzymes
Safety of gene therapy
First Gene Therapy For Heart Failure Offered In Clinical Trials
The ethics of Human Gene Therapy
gene therapy
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