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| Topic: |
Science
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| Number of pages / Number of words: |
7 / 1736 |
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This being the case, beef heart was chosen for the source. The reaction that succinic dehydrogenase facilitates is shown below.
Succinic Dehydrogenase
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COOH-CH2-CH2-COOH + FAD> COOH-CH=CH-COOH + FADH2
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Succinic Acid Fumaric Acid
It has been found that methylene blue can be used for a hydrogen acceptor instead of FAD...
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Reduced methylene blue is clear in color and will be used in our experiment to reveal, relatively, how much succinate has been converted to fumaric acid. The clearer the resulting solution, the more the reaction has progressed. Methylene blue in this case is being used as an indicator. We will be using malonic acid for our competitive inhibitor...
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