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Science
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| Number of pages / Number of words: |
9 / 2305 |
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My proposal is that if we block the proteins Id-1 and Id-3 or prevent them from being expressed, then we will be able to stop the mestastasis of the MCF-7 cells in the body. There are questions that will be raised at the end of the experiment; will angiogenesis stop completely or will it be slower? Will there be any metastasis throughout the body? Can the manipulation of these two proteins lead to a therapy to cure breast cancer? This experiment will be done invivo on nude mice...
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The methods that will be used in the second experiment will be production of polyclonal antibodies, fluorescent labeling, and autoradiography. For the experiment in general, there will be three groups of mice. In the first group, we will block the genes expressing the proteins Id-1 and Id-3; these mice will be our "knockout" mice (Connor 2006)...
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