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| Topic: |
Science
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| Number of pages / Number of words: |
9 / 2347 |
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Mark the sterile 15-ml tubes with “+†and “-“. Use sterile transfer pipette to add 250 ?l ice-cold calcium chloride to the tube. Place the tubes on ice.
2. Use sterile plastic inoculating loop to transfer one or two large colonies of E. coli cells from Starter Plate into your tubes (+ and -)...
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Place the tubes on ice.
2. Use sterile plastic inoculating loop to transfer one or two large colonies of E. coli cells from Starter Plate into your tubes (+ and -).
- Be careful not to transfer any agar from plate along with cell mass.
- Immerse loop in calcium chloride solution and vigorously tap against wall of tube to dislodge bacterial cell mass...
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General points of the essay
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