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Topic: Science
Number of pages / Number of words: 6 / 1414
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Overall blue and red light works best, while green in least effective in the photosynthesis process (Nishio, 2000). The wavelengths that a pigment absorbs, absorption spectra, are determined using a spectrophotometer.

In order to obtain the photosynthetic pigment's absorption spectra the pigments are separated using paper chromatography...


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Paper chromatography is an analytical technique that separates a mixture based on the individual pigment's size, polarity and solubility (Lewis, 2004). The separation of the mixtures involves a stationary phase (the chromatography paper), which a mobile phase (solvent) moves up through. When the mixtures is applied to the paper and allowed to flow with the mobile phase, the different pigments move at different rates (Campbell, 1996)...


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