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M. B., Rousselle, J.-C., Fillion, M.-P., Beaudoin, D. M.,Goiny, M. R., Deniau, J.-M. & Jacob, J. J. C. (1978) Mol. Pharmacol.
14, 50-59.
Whitaker and Seeman used calf caudate homogenate as the substrate for their studies. They were concerned with measuring the specific binding of tritiated LSD ([3H]LSD) in the presence of a variety of competing ligands...
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Specific activity was measured by subtracting non-specific activity (measuring hot ligand added after incubation with cold ligand) from total activity (measuring all hot ligand added). Competition assays were performed with and without presence of APS (apomorphine, phentolamine, and spiperone, all potent catecholamine agonists)...
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