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These STR locations are targeted with sequence- specific primers and are amplified using PCR. The DNA fragments that result are then separated and detected using electrophoresis. There are two common methods of separation and detection, capillary electrophoresis (CE) and gel electrophoresis. Capillary electrophoresis works by electrokinetically injecting the DNA fragments into a thin glass tube filled with polymer...
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Capillary electrophoresis works by electrokinetically injecting the DNA fragments into a thin glass tube filled with polymer. The DNA is pulled through the tube by the application of an electric field, separating the fragments so that that the smaller fragments travel faster through the capillary. The fragments are then detected using fluorescent dyes that were attached to the primers used in PCR...
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