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| Topic: |
Science
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| Number of pages / Number of words: |
4 / 1101 |
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As the DNA is synthesized, nucleotides are added on to the growing chain by the DNA polymerase. However, on occasion a dideoxynucleotide is incorporated into the chain in place of a normal nucleotide, which results in a chain-terminating event. For example if we looked at only the "G" tube, we might find a mixture of the following products:
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Figure 1: An example of the potential fragments that could be produced in the "G" tube...
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The fragments are all different lengths due to the random integration of the ddGTP's (Metzenberg).
The key to this method, is that all the reactions start from the same nucleotide and end with a specific base. Thus in a solution where the same chain of DNA is being synthesized over and over again, the new chain will terminate at all positions where the nucleotide has the potential to be added because of the integration of the dideoxynucleotides (Russell, 2002)...
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