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| Topic: |
Science
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| Number of pages / Number of words: |
8 / 2155 |
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The broths were inoculated with the unknown bacteria cultures and incubated for growth. If fermentation of the sugar molecules was carried out, the pH in the tube would be lowered, and the phenol red would be converted to yellow under the acidic conditions. Thus, the conversion of the originally red broth to yellow signifies a positive test, indicating the bacteria can ferment using either lactose or sucrose...
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If the broth remains red, fermentation on these sugars was not possible and the test is negative. The production of gas by the fermentation was monitored using the inverted Durham tubes. If gas was produced during the fermentation process, the Durham tube would contain a bubble about 2/3 it's size.
The iron agar slant was used to test for the fermentation of glucose and lactose, as well as the production of H2S...
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