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| Topic: |
Science
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| Number of pages / Number of words: |
4 / 1055 |
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One layer only and a small piece of the tape. Keep finger oils off as much as possible. Prepare a master solution of 12mL buffer, 18mL dH2O, and 12mL DPIP. Add 3.5mL of this solution to six cuvettes. In the seventh cuvette, add 2.5mL dH2O and 1mL buffer. Do not add the chloroplasts until the experiment starts...
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Do not add the chloroplasts until the experiment starts.
4. Locate the unboiled and boiled chloroplast suspension prepared by your instructor. Draw up about 1mL of unboiled chloroplast and add 3 drop to every cuvette.
5. Set the lamp 45 cm from your cuvettes. Turn the lamp on but don't put the cuvettes in the light yet...
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