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This approach will be investigated both for biological dosimetry purposes, especially in low-dose contexts (count of abnormal cells, count of abnormalities per cell) and for research purposes (karyotype instability known as tumorigenesis).
Preliminary results demonstrating the feasibility were obtained using hydrodynamic destruction of mitotic cells by capillary flow, high gradient devices and monovariate (DNA quantification) flow karyotyping...
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This approach of cell membrane destruction will be optimised and alternative methods (particularly ultrasonic disintegration) developed. The intracellular staining method of chromosomes with DNA specific fluorochromes will be improved especially for dual parameter (DNA content and base pair composition quantification) intracellular flow karyotype analysis...
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